Cell Signaling Technology

Product Pathways - DNA Damage

GADD45 alpha (D17E8) Rabbit mAb #4632

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 22 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GADD45 alpha Antibody detects endogenous levels of total GADD45 alpha protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Asp129 of human GADD45 alpha.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using GADD45 alpha (D17E8) Rabbit mAb.

Background

GADD45 α, β and γ are an evolutionarily conserved, homologous family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage. GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents (1). GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase (2). GADD45 forms a complex with p21 and Cdc2, and may serve as core for interaction with other cell cycle regulators (3,4).

  1. Wang, X.W. et al. (1999) Proc Natl Acad Sci USA 96, 3706-11.
  2. Yang, Q. et al. (2000) J Biol Chem 275, 36892-8.
  3. Zhao, H. et al. (2000) Exp Cell Res 258, 92-100.
  4. Liebermann, D.A. and Hoffman, B. (2008) J Mol Signal 3, 15.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products