Cell Signaling Technology

Product Pathways - Autophagy Signaling

TMEM49/VMP1 Antibody #4643

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 46 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TMEM49/VMP1 recognizes endogenous levels of total TMEM49/VMP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMEM49/VMP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TMEM49/VMP1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TMEM49/VMP1 (hTMEM49/VMP1-Myc/DDK; +), using TMEM49/VMP1 Antibody.

Background

Vacuole membrane protein 1 (VMP1)/TMEM49 is a transmembrane protein localized to intracellular vacuoles and was discovered as a protein that promotes vacuole formation in acinar cells associated with acute pancreatitis (1). Over-expression of VMP1 promotes vacuole formation and subsequent cell death (1). Subsequent studies have shown that VMP1 expression is induced by starvation and the mTOR inhibitor, rapamycin, and can trigger autophagy (2). VMP1 is targeted, along with LC3, to autophagosome membranes (2). Knockdown of VMP1 can inhibit autophagosome formation (2). VMP1 interacts with Beclin-1, a key autophagy protein that activates the Class III PI3 kinase Vps34, which is regulated by a large network of associated proteins (3). VMP1 functions in the degradation and clearance of zymogen-containing vacuoles during experimental pancreatitis (4). During this process, VMP1 interacts with the ubiquitin protease USP9X, suggesting a possible functional link between the molecular machinery of autophagy and the ubiquitin pathway. Orthologues of VMP1 have been reported in C. elegans (known as EPG-3), Drosophila (known as TANGO-5), and Dictyostelium, and have been shown to play a role in membrane trafficking, orgenelle organization, and autophagy (5-7).

  1. Dusetti, N.J. et al. (2002) Biochem Biophys Res Commun 290, 641-9.
  2. Ropolo, A. et al. (2007) J Biol Chem 282, 37124-33.
  3. Kang, R. et al. (2011) Cell Death Differ 18, 571-80.
  4. Grasso, D. et al. (2011) J Biol Chem 286, 8308-24.
  5. Tian, Y. et al. (2010) Cell 141, 1042-55.
  6. Bard, F. et al. (2006) Nature 439, 604-7.
  7. Calvo-Garrido, J. et al. (2008) Mol Biol Cell 19, 3442-53.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products