Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

GOPC Antibody #4646

Applications Reactivity Sensitivity MW (kDa) Source
W IP H R (M) Transfected Only 59 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GOPC Antibody detects transfected levels of total GOPC protein. The antibody also detects endogenous levels of GOPC following IP enrichment.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met266 of human GOPC protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, untransfected or transfected with human GOPC, using GOPC Antibody.

Background

GOPC (FIG) was originally identified as a Golgi-associated, PDZ domain containing protein. It has two coiled-coil domains (CC1 and CC2) located in the amino-terminal region and a PDZ domain in the carboxy-terminal region (1). The CC2 domain and its adjacent linker region mediate the association of GOPC with the golgi protein golgin-160 and the Q-SNARE protein syntaxin 6 (1,2). The PDZ domain of GOPC interacts with the carboxy terminus of target proteins to mediate target protein vesicular trafficking and surface expression (3-6). Fusion of the corresponding GOPC gene with the ROS tyrosine kinase oncogene has been detected in some glioblastomas. The resulting GOPC-ROS fusion protein is targeted to the golgi apparatus where a constitutively activate ROS tyrosine kinase can mediate tumor formation (7,8).

  1. Charest, A. et al. (2001) J Biol Chem 276, 29456-65.
  2. Hicks, S.W. and Machamer, C.E. (2005) J Biol Chem 280, 28944-51.
  3. Cheng, J. et al. (2002) J Biol Chem 277, 3520-9.
  4. He, J. et al. (2004) J Biol Chem 279, 50190-6.
  5. Wente, W. et al. (2005) J Biol Chem 280, 32419-25.
  6. Ito, H. et al. (2006) Biochem J 397, 389-98.
  7. Charest, A. et al. (2003) Proc Natl Acad Sci USA 100, 916-21.
  8. Charest, A. et al. (2006) Cancer Res 66, 7473-81.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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