Product Pathways - Cell Cycle / Checkpoint
Cyclin A (BF683) Mouse mAb #4656
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W F | H | Endogenous | 55 | Mouse IgE |
Applications Key:
W=Western Blotting
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cyclin A (BF683) Mouse mAb detects endogenous levels of cyclin A and does not cross-react with other cyclins.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a recombinant human cyclin A protein.
Western Blotting
Western analysis of extracts from Hela cells that were untreated, treated with doxorubicin (0.5uM, 24 hours), or with nocodazole (50ng/ml, 24 hours), using Cyclin A (BF683) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells, using Cyclin A (BF683) Mouse mAb versus propidium iodide (DNA content) . The boxed population indicates Cyclin A-positive cells.
Background
While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of over-expressed cyclin A to accellerate the G1 to S transition causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5).
- Pagano, M. et al. (1992) EMBO. J. 11, 961-971.
- Resnitzky, D. et al. (1995) Mol. Cell. Biol. 15, 4347-4352.
- d'Urso, G. et al. (1990) Science 250, 786-791.
- Zindy, F. et al. (1992) Biochem. Biophys. Res. Commun. 182, 1144-1154.
- Furuno, N. et al. (1999) J. Cell. Biol. 147, 295-306.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
