Product Pathways - Cell Cycle / Checkpoint
Cyclin A2 (BF683) Mouse mAb #4656
PhosphoSitePlus® protein, site, and accession data: CCNA2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W F | H | Endogenous | 55 | Mouse IgG2b |
Applications Key:
W=Western Blotting
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4656:
- Flow, Western Blotting
Specificity / Sensitivity
Cyclin A2 (BF683) Mouse mAb detects endogenous levels of cyclin A2 and does not cross-react with other cyclins or Cyclin A1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human cyclin A2 protein.
Western Blotting
Western analysis of extracts from Hela cells that were untreated, treated with doxorubicin (0.5 µM, 24 hours), or with nocodazole (50 ng/ml, 24 hours), using Cyclin A2 (BF683) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells, using Cyclin A2 (BF683) Mouse mAb versus propidium iodide (DNA content). Note decrease in expression of Cyclin A during late mitosis (arrow).
Background
While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of over-expressed cyclin A to accellerate the G1 to S transition causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5).
- Pagano, M. et al. (1992) EMBO. J. 11, 961-971.
- Resnitzky, D. et al. (1995) Mol. Cell. Biol. 15, 4347-4352.
- d'Urso, G. et al. (1990) Science 250, 786-791.
- Zindy, F. et al. (1992) Biochem. Biophys. Res. Commun. 182, 1144-1154.
- Furuno, N. et al. (1999) J. Cell. Biol. 147, 295-306.
Application References
- Chang, S.F. et al. (2008) Proc Natl Acad Sci U S A 105, 3927-32. Applications: Western Blotting
- Tagoug, I. et al. (2011) PLoS One 6, e22641. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
