Product Pathways - Cytoskeletal Signaling
Vav1 (D45G3) Rabbit mAb #4657
PhosphoSitePlus® protein, site, and accession data: VAV1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H (Mk) | Endogenous | 95 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Vav1 (D45G3) Rabbit mAb recognizes endogenous levels of total Vav1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy terminus of human Vav1 protein.
Western Blotting
Western blot analysis of extracts from Jurkat, Raji and K-562 cells using Vav1 (D45G3) Rabbit mAb.
IP
Immunoprecipitation of Vav1 from Jurkat cells using Vav1 (D45G3) Rabbit mAb. Western blot was performed using the same antibody.
Background
Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).
- Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
- Crespo, P. et al. (1997) Nature 385, 169-172.
- Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
