Cell Signaling Technology

Product Pathways - DNA Damage

p73 Antibody #4662

Applications Reactivity Sensitivity MW (kDa) Source
W H (M) (Mk) Transfected Only 80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

p73 Antibody detects transfected levels of total p73. Because endogenous levels of p73 protein are low, it may be difficult to detect endogenous p73.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human p73. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1376 cells, untreated or pervanadate-treated (1 mM, 20 minutes), using Phospho-p73 (Tyr99) Antibody #4665 (upper), p73 Antibody (middle), or p63 (4A4) Mouse mAb #4894 antibody (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells untransfected (lane 1) or transfected with p73 DNA (lanes 2 and 3). Overexpression of p73 can be specifically blocked by co-transfection with p73 siRNA #6371 (lane 3). p73 was detected using p73 Antibody and p42 was detected using p42 MAPK Antibody #9108. The p73 Antibody confirms silencing of exogenous p73 expression and the p42 MAPK Antibody is used to control for loading and siRNA specificity.

Background

The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator (1-3). Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix (4). DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 (5). Another report, however, indicates that p300 does not acetylate full length p73 in vivo (6).

  1. Kaghad, M. et al. (1997) Cell 90, 809-819.
  2. Jost, C.A. et al. (1997) Nature 389, 191-194.
  3. De Laurenzi, V. D. et al. (1999) Cell Death Differ. 6, 389-390.
  4. Ben-Yehoyada, M. et al. (2003) J. Biol. Chem. 278, 34475-34482.
  5. Costanzo, A. et al. (2002) Mol. Cell 9, 175-186.
  6. Zeng, X. et al. (2001) J. Biol. Chem. 276, 48-52.

Application References

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Companion Products

Because endogenous levels of p73 protein are low, it may be difficult to detect endogenous p73 by western blot using p73 Antibody #4662.

For Research Use Only. Not For Use In Diagnostic Procedures.

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