Product Pathways - DNA Damage
p73 Antibody #4662
|4662S||100 µl (10 western blots)||---||In Stock||---|
|4662||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Mouse, Monkey.
Specificity / Sensitivity
p73 Antibody detects transfected levels of total p73. Because endogenous levels of p73 protein are low, it may be difficult to detect endogenous p73.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human p73. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HT-1376 cells, untreated or pervanadate-treated (1 mM, 20 minutes), using Phospho-p73 (Tyr99) Antibody #4665 (upper), p73 Antibody (middle), or p63 (4A4) Mouse mAb #4894 antibody (lower).
Western blot analysis of extracts from HeLa cells untransfected (lane 1) or transfected with p73 DNA (lanes 2 and 3). Overexpression of p73 can be specifically blocked by co-transfection with p73 siRNA #6371 (lane 3). p73 was detected using p73 Antibody and p42 was detected using p42 MAPK Antibody #9108. The p73 Antibody confirms silencing of exogenous p73 expression and the p42 MAPK Antibody is used to control for loading and siRNA specificity.
The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator (1-3). Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix (4). DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 (5). Another report, however, indicates that p300 does not acetylate full length p73 in vivo (6).
- Kaghad, M. et al. (1997) Cell 90, 809-819.
- Jost, C.A. et al. (1997) Nature 389, 191-194.
- De Laurenzi, V. D. et al. (1999) Cell Death Differ. 6, 389-390.
- Ben-Yehoyada, M. et al. (2003) J. Biol. Chem. 278, 34475-34482.
- Costanzo, A. et al. (2002) Mol. Cell 9, 175-186.
- Zeng, X. et al. (2001) J. Biol. Chem. 276, 48-52.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 4665 Phospho-p73 (Tyr99) Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.