Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-p73 (Tyr99) Antibody #4665

Applications Reactivity Sensitivity MW (kDa) Source
W H (M) (R) (Mk) Endogenous 80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-p73 (Tyr99) Antibody detects endogenous levels of p73 only when phosphorylated at tyrosine 99. This antibody cross-reacts with p63 when phosphorylated at tyrosine 149.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr99 of human p73. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1376 cells, untreated or pervanadate-treated ( 1 mM, 20 minutes), using Phospho-p73 (Tyr99) Antibody (upper), p73 Antibody #4662 (middle), or p63 (4A4) Mouse mAb #4894 (lower).

Background

The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator (1-3). Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix (4). DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 (5). Another report, however, indicates that p300 does not acetylate full length p73 in vivo (6).

While the sequences surrounding p73 Tyr99 and p63 Tyr149 are very similar, only p73 co-localizes with c-Abl following gamma irradiation, suggesting that p63 is not a c-Abl substrate (7).

  1. Kaghad, M. et al. (1997) Cell 90, 809-819.
  2. Jost, C.A. et al. (1997) Nature 389, 191-194.
  3. De Laurenzi, V. D. et al. (1999) Cell Death Differ. 6, 389-390.
  4. Ben-Yehoyada, M. et al. (2003) J. Biol. Chem. 278, 34475-34482.
  5. Costanzo, A. et al. (2002) Mol. Cell 9, 175-186.
  6. Zeng, X. et al. (2001) J. Biol. Chem. 276, 48-52.
  7. Hamer, G. et al. (2001) Oncogene 20, 4298-4304.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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