Cell Signaling Technology

Product Pathways - DNA Damage

WRN (8H3) Mouse mAb #4666

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Endogenous 200 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

WRN (8H3) Mouse mAb detects endogenous levels of total WRN protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full length human recombinant WRN protein.

Western Blotting

Western Blotting

Western blot analysis of HeLa, Jurkat, and Ramos cell extracts using WRN (8H3) Mouse mAb.

Background

The RecQ family of DNA and RNA helicases is a family of enzymes that has been shown to be important to genome integrity (1). Members of this family function in several DNA repair processes including double strand break repair, homologous recombination, and re-initiation of DNA replication at stalled replication forks (2,3). Mutations in RecQ helicase family members results in syndromes that display varying types of chromosomal abnormalities and overall genomic instability (1). WRN is a member of the RecQ family that has been identified as the gene underlying Werner’s syndrome; an autosomal recessive disorder characterized by premature aging and predisposition to cancer (4). Cells from Werner’s Syndrome patients exhibit genomic instability that is associated with deficient DNA repair activity (5).

  1. Cobb, J.A. et al. (2002) FEBS Lett 529, 43-8.
  2. Ouyang, K.J. et al. Mech Ageing Dev 129, 425-40.
  3. Kaneko, H. et al. (2004) Adv Biophys 38, 45-64.
  4. Epstein, C.J. et al. (1966) Medicine (Baltimore) 45, 177-221.
  5. Salk, D. et al. (1985) Adv Exp Med Biol 190, 541-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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