Product Pathways - MAPK Signaling
Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H M R Hm | 46, 54 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit Monoclonal Antibody detects endogenous levels of p46 and p54 SAPK/JNK only when phosphorylated at Thr183 and Tyr185. This antibody does not recognize phosphorylated p44/42 MAPK or p38 MAP kinase.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK.
Western Blotting
Western blot analysis of purified MAPK phospho-proteins or extracts from NIH/3T3 cellstreated with UV light and PDGF, using Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mAb (upper), Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (middle), and Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 (lower).
Western Blotting
Western blot analysis of extracts from 293 cells and NIH/3T3 cells, untreated or UV-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb (upper) or SAPK/JNK (56G8) Rabbit mAb (lower).
Background
The stress-activated protein kinase/Jun-amino-terminal kinase (SAPK/JNK) is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4-7, which then phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4-7 can be activated by a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family (4). There are three SAPK/JNK genes with further diversification resulting from alternative splicing (3). Active SAPK/JNK dimers can translocate to the nucleus to regulate transcription through its effects on c-Jun, ATF-2 and other transcription factors (3,5).
- Davis, R.J. (1999) Biochem. Soc. Symp. 64, 1-12.
- Ichijo, H. (1999) Oncogene 18, 6087-6093.
- Kyriakis, J.M. and Avruch, J. (2001) Physiol. Rev. 81, 807-869.
- Kyriakis, J.M. (1999) J. Biol. Chem. 274, 5259-5262.
- Leppa, S. and Bohmann, D. (1999) Oncogene 18, 6158-6162.
- Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
