Cell Signaling Technology

Product Pathways - MAPK Signaling

JNK2 Antibody #4672

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Hm Endogenous 54 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hm=Hamster
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

JNK2 Antibody detects endogenous levels of JNK2. This antibody does not recognize p38 MAP kinase, p44/42MAP kinase or JNK1.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) derived from human JNK2. Antibodies are purified by protein A and peptide affinity chromatography

Western Blotting

Western Blotting

Western blot analysis of extracts from 293, C2C12, NIH/3T3, PC12, and COS cells, using JNK2 Antibody.

Background

The stress-activated protein kinase/Jun-amino-terminal kinase (SAPK/JNK) is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4-7, which then phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4-7 can be activated by a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family (4). There are three SAPK/JNK genes with further diversification resulting from alternative splicing (3). Active SAPK/JNK dimers can translocate to the nucleus to regulate transcription through its effects on c-Jun, ATF-2 and other transcription factors (3,5).

  1. Davis, R.J. (1999) Biochem. Soc. Symp. 64, 1-12.
  2. Ichijo, H. (1999) Oncogene 18, 6087-6093.
  3. Kyriakis, J.M. and Avruch, J. (2001) Physiol. Rev. 81, 807-869.
  4. Kyriakis, J.M. (1999) J. Biol. Chem. 274, 5259-5262.
  5. Leppa, S. and Bohmann, D. (1999) Oncogene 18, 6158-6162.
  6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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