Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

BMP4 (6B7) Mouse mAb #4680

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Transfected Only 47 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

BMP4 (6B7) Mouse mAb detects transfected levels of total BMP4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a BMP4 fusion protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, either mock transfected or transfected with a human BMP4 construct, using BMP4 (6B7) Mouse mAb.

Background

Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belongs to the TGF-β superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the c-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).

BMP4 plays an essential role during development. Mouse embyros deficient in BMP4 die around the time of gastrulation (5). BMP4 regulates stem cell maintenance and differentiation in various systems (6-8).

  1. Wang, E.A. et al. (1988) Proc Natl Acad Sci USA 85, 9484-8.
  2. Wozney, J.M. et al. (1988) Science 242, 1528-34.
  3. Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
  4. Nohe, A. et al. (2004) Cell Signal 16, 291-9.
  5. Dunn, N.R. et al. (1997) Dev Biol 188, 235-47.
  6. Nakayama, N. et al. (2000) Blood 95, 2275-83.
  7. Solmesky, L.J. et al. (2009) Stem Cells Dev 18, 1283-92.
  8. Goldman, O. et al. (2009) Stem Cells 27, 1750-9.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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