Cell Signaling Technology

Product Pathways - Development

TLE1/2/3/4 Antibody #4681

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk Dm (R) (X) (Z) Endogenous 78-90 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster  X=Xenopus  Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TLE1/2/3/4 Antibody detects endogenous levels of TLE1, TLE2, TLE3, and TLE4 proteins. Based on homology it should also recognize TLE1 and TLE2 of Zebra fish, TLE2 of Xenopus, and Groucho of Drosophila.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp760 of human TLE1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell types using TLE1/2/3/4 Antibody.

Background

Transducin-like enhancer of split proteins (TLE1, TLE2, TLE3, TLE4, and TLE6) are mammalian homologs of Drosophila Groucho (1). TLEs contain several WD-repeats implicated in protein-protein interaction (2,3). TLEs are transcriptional co-repressors that bind to many transcription factors such as LEF1, Runx1, Oct-1, hepatocyte nuclear factor 3-β as well as histone H3 (4-7). TLEs are differentially expressed during animal development and may have overlapping as well as distinct functions (8).

  1. Stifani, S. et al. (1992) Nat. Genet. 2, 119-127.
  2. Chen, G. and Courey, A.J. (2000) Gene 249, 1-16.
  3. Jennings, B.H. et al. (2006) Mol. Cell 22, 645-655.
  4. Levanon, D. et al. (1998) Proc. Natl. Acad. Sci. U S A 95, 11590-11595.
  5. Malin, S. et al. (2005) Nucleic Acids Res. 33, 4618-4625.
  6. Wang, J.C. et al. (2000) J. Biol. Chem. 275, 18418-18423.
  7. Palaparti, A. et al. (1997) J. Biol. Chem. 272, 26604-26610.
  8. Gasperowicz, M. and Otto, F. (2005) J. Cell Biochem. 95, 670-687.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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