Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Chronophin/PDXP (C85E3) Rabbit mAb #4686

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Hm Mk B Endogenous 31 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Chronophin/PDXP (C85E3) Rabbit mAb detects endogenous levels of total chronophin/PDXP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant mouse MBP-chronophin.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Chronophin/PDXP (C85E3) Rabbit mAb.

Background

Cofilin is a conserved actin-severing protein required for processes that rely on actin dynamics, including cytokinesis and cell motility (reviewed in 1). Regulation of actin dynamics requires the controlled cycling between the phosphorylated and unphosphorylated forms of cofilin (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at the conserved amino-terminal Ser3 of cofilin (3,4). Slingshot (SSH) phosphatase, for which there have been three mammalian isoforms identified, dephosphorylates cofilin in vivo (5). Chronophin (CIN, PDXP) is a haloacid dehalogenase phosphatase that also dephosphorylates cofilin. Alteration of CIN activity through overexpression of either the wildtype or phosphatase-inactive mutant CIN interferes with actin dynamics, cell morphology and cytokinesis (6).

  1. Carlier, M.F. et al. (1999) J. Biol. Chem. 274, 33827-33830.
  2. Huang, T.Y. et al. (2006) Curr. Opin. Cell Biol. 18, 26-31.
  3. Arber, S. et al. (1998) Nature 393, 805-809.
  4. Toshima, J. et al. (2001) J. Biol. Chem. 276, 31449-31458.
  5. Niwa, R. et al. (2002) Cell 108, 233-246.
  6. Gohla, A. et al. (2005) Nat. Cell Biol. 7, 21-29.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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