Cell Signaling Technology

Product Pathways - Development

DKK1 Antibody #4687

Applications Reactivity Sensitivity MW (kDa) Source
W H Transfected Only 30, 35 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DKK1 Antibody detects transfected levels of DKK1 protein. This antibody does not cross-react with DKK2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln75 of human DKK1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing DKK1, using DKK1 Antibody.

Background

Dickkopf (DKK) family proteins consist of four members DKK1, DKK2, DKK3 and DKK4 that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6).

DKK1 was initially identified as an inducer of head formation in Xenopus embryos (7) and plays an important role in the regulation of bone mass (8-10). Increased levels of DKK1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers (11,12), while DKK1 expression is decreased in malignant melanoma and colorectal cancers (13,14).

  1. Mao, B. et al. (2001) Nature 411, 321-5.
  2. Niehrs, C. (2006) Oncogene 25, 7469-81.
  3. Krupnik, V.E. et al. (1999) Gene 238, 301-13.
  4. Monaghan, A.P. et al. (1999) Mech Dev 87, 45-56.
  5. Mao, B. et al. (2002) Nature 417, 664-7.
  6. Davidson, G. et al. (2002) Development 129, 5587-96.
  7. Glinka, A. et al. (1998) Nature 391, 357-62.
  8. Baron, R. and Rawadi, G. (2007) Curr Osteoporos Rep 5, 73-80.
  9. MacDonald, B.T. et al. (2007) Bone 41, 331-9.
  10. Diarra, D. et al. (2007) Nat Med 13, 156-63.
  11. Forget, M.A. et al. (2007) Br J Cancer 96, 646-53.
  12. Yamabuki, T. et al. (2007) Cancer Res 67, 2517-25.
  13. Kuphal, S. et al. (2006) Oncogene 25, 5027-36.
  14. Aguilera, O. et al. (2006) Oncogene 25, 4116-21.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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