Product Pathways - Cell Cycle / Checkpoint
cdc25C (5H9) Rabbit mAb #4688
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 60, 80 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Protocols
- 4688:
- Western Blotting
Specificity / Sensitivity
cdc25C (5H9) Rabbit mAb detects endogenous levels of total cdc25C protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with GST-cdc25C full length fusion protein.
Western Blotting
Western blot analysis of extracts from HeLa, 293 and K562 cells, using cdc25C (5H9) Rabbit mAb.
Western Blotting
Western Blot analysis of HT29 cell extracts untreated, nocodazole-treated, and lambda phosphotase-treated using Phospho-cdc25C (Thr48), #9527 (upper), and cdc25C (5H9) Rmab (lower).
Background
cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).
- Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
- Peng, C.Y. et al. (1997) Science 277, 1501-1505.
- Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
- Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
- Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
Application References
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Companion Products
- 4901 Phospho-cdc25C (Ser216) (63F9) Rabbit mAb
- 9528 Phospho-cdc25C (Ser216) Antibody
- 9527 Phospho-cdc25C (Thr48) Antibody
- 9529 Phospho-cdc25C (Ser198) Antibody
- 3652 cdc25A Antibody
- 9525 cdc25B Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
