Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

DKK2 (N70) Antibody #4689

Applications Reactivity MW (kDa) Source
W H 28, 32 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

DKK2 (N70) Antibody detects transfected levels of human DKK2 protein. This antibody does not cross-react with DKK1.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Asn70 of human DKK2. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing DKK2, using DKK2 (N70) Antibody.

Background

Dickkopf (DKK) family proteins consist of four members DKK1, DKK2, DKK3 and DKK4 that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6).

DKK2 plays a role in osteoblast terminal differentiation and formation of mineralized matrix and is required for differentiation of non-keratinizing stratified epithelium from the corneal epithelial progenitors (7,8).

  1. Mao, B. et al. (2001) Nature 411, 321-5.
  2. Niehrs, C. (2006) Oncogene 25, 7469-81.
  3. Krupnik, V.E. et al. (1999) Gene 238, 301-13.
  4. Monaghan, A.P. et al. (1999) Mech Dev 87, 45-56.
  5. Mao, B. et al. (2002) Nature 417, 664-7.
  6. Davidson, G. et al. (2002) Development 129, 5587-96.
  7. Li, X. et al. (2005) Nat Genet 37, 945-52.
  8. Mukhopadhyay, M. et al. (2006) Development 133, 2149-54.

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