Cell Signaling Technology

Product Pathways - Development

SFRP1 Antibody #4690

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 35 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SFRP1 Antibody detects endogenous levels of total SFRP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly48 of human SFRP1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from U118MG and SK-MEL-28 cells using SFRP1 Antibody.

Background

Secreted Frizzled-related proteins (SFRPs) display homology and structural similarity to the extracellular cysteine-rich Wnt-binding domain of the G protein-coupled receptor Frizzled (1,2). To date, five distinct SFRPs (SFRP1 to 5) have been found in mammalian cells. These secreted proteins typically act as antagonists to Wnt signaling by directly binding and inhibiting Wnt proteins, or by binding Frizzled to block Wnt protein interaction with the receptor (3). The various SFRPs bind and regulate Wnt proteins differentially; these proteins also display distinct expression patterns as they play important roles in regulating development (4-7). SFRP proteins appear to act as tumor suppressors, with loss of expression or function correlating with many invasive forms of cancer. Deletion of the corresponding SFRP1 gene and promoter hypermethylation leading to gene silencing has been reported in a number of cancers. Abnormal expression of SRFP1 and other Wnt signaling proteins is associated with some cases of retinitis pigmentosa (reviewed in 8).

  1. Dann, C.E. et al. (2001) Nature 412, 86-90.
  2. Kawano, Y. and Kypta, R. (2003) J Cell Sci 116, 2627-34.
  3. Bafico, A. et al. (1999) J Biol Chem 274, 16180-7.
  4. Galli, L.M. et al. (2006) Dev Dyn 235, 681-90.
  5. Wawrzak, D. et al. (2007) Biochem Biophys Res Commun 357, 1119-23.
  6. Hu, E. et al. (1998) Biochem Biophys Res Commun 247, 287-93.
  7. Mao, C. et al. (2000) Arterioscler Thromb Vasc Biol 20, 43-51.
  8. Bovolenta, P. et al. (2008) J Cell Sci 121, 737-46.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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