Cell Signaling Technology

Product Pathways - Development

BMP7 Antibody #4693

Applications Reactivity Sensitivity MW (kDa) Source
W IP M Transfected Only 49 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BMP7 Antibody detects transfected levels of total mouse BMP7 protein. It only recognizes the BMP7 precursor protein (49 kDa), not the mature secreted from (16 kDa).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly99 of mouse BMP7. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, mock transfected or transfected with mouse BMP7, using BMP7 Antibody.

Background

Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belongs to the TGF-β superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the c-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).

BMP7, also known as osteogenic protein-1 (OP-1), is found to be upregulated in some cancer cells (5-7), and may play a role in cancer metastasis (7-9).

  1. Wang, E.A. et al. (1988) Proc Natl Acad Sci USA 85, 9484-8.
  2. Wozney, J.M. et al. (1988) Science 242, 1528-34.
  3. Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
  4. Nohe, A. et al. (2004) Cell Signal 16, 291-9.
  5. Yang, S. et al. (2005) Cancer Res 65, 5769-77.
  6. Alarmo, E.L. et al. (2006) Genes Chromosomes Cancer 45, 411-9.
  7. Motoyama, K. et al. (2008) Ann Surg Oncol 15, 1530-7.
  8. Buijs, J.T. et al. (2007) Cancer Res 67, 8742-51.
  9. Buijs, J.T. et al. (2007) Am J Pathol 171, 1047-57.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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