Cell Signaling Technology

Product Pathways - MAPK Signaling

MEK1/2 (L38C12) Mouse mAb #4694

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC F H M R Mk Endogenous 45 Mouse IgG1

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MEK1/2 (L38C12) Mouse mAb detects endogenous levels of total MEK1/2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full length MEK1/2 proteins.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, PC12 and COS cells, using MEK1/2 (L38C12) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using MEK1/2 (L38C12) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MEK1/2 (L38C12) Mouse mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using MEK1/2 (L38C12) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using MEK1/2 (L38C12) Mouse mAb compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescence images of untreated HeLa cells labeled with MEK1/2 (L38C12) Mouse mAb (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

  1. Crews, C.M. et al. (1992) Science 258, 478-480.
  2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
  4. Cowley, S. et al. (1994) Cell 77, 841-852.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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