Cell Signaling Technology

Product Pathways - Adhesion

Integrin β3 Antibody #4702

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk B Endogenous 97, 110, 130 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Integrin beta3 Antibody detects endogenous levels of total integrin beta3. This antibody does not cross-react with other integrin isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to human and mouse integrin beta3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HUVEC, BAEC, FDCP1, RLME, Raw and HeLa cells using Integrin beta3 Antibody.

Background

Integrins are heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlaping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). αIIβ3 and αVβ3 are the two β3 containing integrins which are prominently expressed in hematopoietic cells and angiogenic endothelic cells and perform adhesive functions in hemostasis, wound healing and angiogenesis (1,4). Tyr773 and Tyr785 (usually referred to as Tyr747 and Tyr759 based on the chicken sequence) are phosphorylated upon ligand binding (5). Phosphorylation of these tyrosine residues is required for certain ligand-induced signaling (6). Thr779 (corresponding to Thr753 of the chicken sequence) of integrin β3 in the platelet specific αIIβ3 is phosphorylated by PKD and/or Akt, which may modulate integrin association with other signaling molecules (7).

  1. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
  4. Shattil, S.J. et al. (1995) Thromb. Haemost. 74, 149-155.
  5. Blystone, S.D. (2002) J. Biol. Chem. 277, 46886-46890.
  6. Butler, B. et al. (2003) J. Biol. Chem. 278, 5264-5270.
  7. Kirk, R.I. et al. (2000) J. BIol. Chem. 275, 30901-30906.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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