Cell Signaling Technology

Product Pathways - Adhesion

Integrin α5 Antibody #4705

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk Endogenous 150 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Integrin α5 Antibody detects endogenous levels of total integrin α5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser458 of human integrin α5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549, NIH/3T3 and COS cell lines using Integrin α5 Antibody.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).Integrin α5/β1 is involved in multiple biological processes including embryonic development, angiogenesis and tumor metastasis (4,5). By interaction with its fibronectin ligand, α5/β1 transduces signals that regulate cell adhesion, migration, matrix assembly and cytoskeletal organization (6).

  1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
  4. Kabir-Salmani, M. et al. (2004) Mol Hum Reprod 10, 91-7.
  5. Kim, S. et al. (2000) J Biol Chem 275, 33920-8.
  6. Miyamoto, S. et al. (1995) J Cell Biol 131, 791-805.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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