Cell Signaling Technology

Product Pathways - Adhesion

Integrin β1 Antibody #4706

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 115, 135 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Integrin β1 Antibody detects endogenous levels of total integrin β1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro680 of human integrin β1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Integrin β1 Antibody.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).The β1 subfamily includes 12 distinct integrin proteins that bind to different extracellular matrix molecules (4). Control of extracellular integrin binding influences cell adhesion and migration, while intracellular signaling messages relayed by the β1 cytoplasmic tail help to regulate cell proliferation, cytoskeletal reorganization, and gene expression (4). Research studies have implicated β1 integrin in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis (4,5).

  1. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
  4. Brakebusch, C. and Fässler, R. (2005) Cancer Metastasis Rev. 24, 403-411.
  5. Mettouchi, A. and Meneguzzi, G. (2006) Eur. J. Cell Biol. 85, 243-247.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products