Cell Signaling Technology

Product Pathways - Adhesion

Integrin β1 Antibody #4706

Applications Reactivity MW (kDa) Source
W H M R Mk Hm 115, 135 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hm=Hamster
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Integrin β1 Antibody detects endogenous levels of total integrin β1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Pro680 of human integrin β1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Integrin β1 Antibody.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlaping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2).The β1 subfamily includes 12 distinct intergrin proteins that bind to different extracellular matrix molecules (4). Control of extracellular integrin binding influences cell adhesion and migration, while intracellular signaling messages relayed by the β1 cytoplasmic tail helps to regulate cell proliferation, cytoskeletal reorganization and gene expression (4). β1 integrin is implicated in various activities including embryonic development, blood vessel, skin, bone and muscle formation, as well as tumor metastasis and angiogenesis (4,5).

  1. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
  4. Brakebusch, C. and Fassler, R. (2005) Cancer Metastasis Rev. 24, 403-411.
  5. Mettouchi, A. and Meneguzzi, G. (2006) Eur. J. Cell Biol. 85, 243-247.

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