Cell Signaling Technology

Product Pathways - Adhesion

Integrin β4 Antibody #4707

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 210 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Integrin β4 Antibody detects endogenous levels of total integrin β4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1812 of human integrin β4 protein. Antibodies are purified peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Integrin β4 Antibody.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2).Integrin β4 pairs with integrin α6 on the cell surface membrane to form the integrin α6β4 heterodimer, an important laminin receptor that is essential for hemidesmosome formation and the support of stable adhesions between basal epithelial cells and the basement membrane (4,5). Integrin β4 is an important component in several growth factor induced signaling pathways that are involved in tumorigenesis and invasive cell growth (6,7).

  1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
  4. Schaapveld, R.Q. et al. (1998) J Cell Biol 142, 271-84.
  5. Litjens, S.H. et al. (2006) Trends Cell Biol 16, 376-83.
  6. Guo, W. et al. (2006) Cell 126, 489-502.
  7. Bertotti, A. et al. (2006) J Cell Biol 175, 993-1003.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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