Cell Signaling Technology

Product Pathways - Adhesion

Integrin β5 Antibody #4708

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H Endogenous 90 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Integrin β5 Antibody detects endogenous levels of total integrin β5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys789 of human integrin β5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 and A172 cell lines using Integrin β5 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A172 cells using Integrin β5 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins having distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2).The αVβ5 integrin is expressed in various tissues and cell types, including endothelia, epithelia and fibroblasts (4). It plays a role in matrix adhesion to VN, FN, SPARC and bone sialoprotein (5) and functions in the invasion of gliomas and metastatic carcinoma cells (6,7). αVβ5 integrin plays a major role in growth-factor-induced tumor angiogenesis, where cooperative signaling by the αVβ5 integrin and growth factors regulates endothelial cell proliferation and survival (8).

  1. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
  2. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
  4. Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111.
  5. Plow, E.F. et al. (2000) J. Biol. Chem. 275, 21785-21788.
  6. Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166.
  7. Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072.
  8. Alghisi, G.C. and Rüegg, C. (2006) Endothelium 13, 113-135.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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