Product Pathways - Apoptosis
TRAF1 (1F3) Rat mAb #4710
PhosphoSitePlus® protein, site, and accession data: TRAF1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R | Endogenous | 50 | Rat IgG2a |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
TRAF1 (1F3) Rat mAb detects endogenous levels of total TRAF1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a TRAF1-GST fusion protein and purified by protein G affinity chromatography.
Western Blotting
Western blot analysis of extracts from Raji and SR cells, using TRAF1 (1F3) Rat mAb.
IHC-P (paraffin)
Immunohisotchemical analysis of paraffin-embedded human tonsil using TRAF1 (1F3) Rat mAb.
IF-IC
Immunofluorescent staining of Raji cells showing cytoplasmic staining, using TRAF1 (1F3) Rat mAb.
Background
TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.
- Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
- Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
- Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
- Rothe, M. et al. (1994) Cell 78, 681-692.
Application References
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Companion Products
- 4724 TRAF2 (C192) Antibody
- 4729 TRAF3 Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4715 TRAF1 (45D3) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
