Product Pathways - Apoptosis / Autophagy
TRAF1 (1F3) Rat mAb #4710
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R | Endogenous | 50 | Rat | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TRAF1 (1F3) Rat mAb detects endogenous levels of total TRAF1 protein.
Source / Purification
Rat monoclonal antibodies were prepared by immunizing rats with a TRAF1-GST fusion protein and purified by protein G affinity chromatography.
Western Blotting
Western blot analysis of extracts from Raji and SR cells, using TRAF1 (1F3) Rat mAb.
IHC-P (paraffin)
Immunohisotchemical analysis of paraffin-embedded human tonsil, using TRAF1 (1F3) Rat mAb.
IF-IC
Immunofluorescent staining of Raji cells showing cytoplasmic staining, using TRAF1 (1F3) Rat mAb.
Background
TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation and stress responses.
- Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
- Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
- Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
- Rothe, M. et al. (1994) Cell 78, 681-692.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
