Product Pathways - Apoptosis / Autophagy
TRAF1 (45D3) Rabbit mAb #4715
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H (Mk) | Endogenous | 50 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TRAF1 (45D3) Rabbit mAb detects endogenous levels of total TRAF1 protein.
Source / Purification
Moncoclonal antibodies are produced by immunzing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding cysteine 57 of human TRAF1.
Western Blotting
Western blot analysis of extracts from Raji and SR cells, using TRAF1 (45D3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human tonsil, using TRAF1 (45D3) Rabbit mAb preincubated with a control peptide (left) or TRAF1 Blocking Peptide #1066 (right).
Flow Cytometry
Flow cytometric analysis of Raji cells, using TRAF1 (45D3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Immunofluorescent analysis of Raji cells showing cytomplasmic localization of TRAF1, using TRAF1 (45D3) Rabbit mAb.
Background
TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation and stress responses.
- Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
- Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
- Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
- Rothe, M. et al. (1994) Cell 78, 681-692.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4710 TRAF1 (1F3) Rat mAb
- 4724 TRAF2 Antibody (Human Specific)
- 4729 TRAF3 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1066 TRAF1 Blocking Peptide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
