Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

RACK1 Antibody #4716

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Dm Z Endogenous 32 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Dm=D. melanogaster  Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RACK1 Antibody recognizes endogenous levels of total RACK1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human RACK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using RACK1 Antibody.

Background

The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the β subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCβII and other classical PKC isoforms (1). RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways (reviewed in 2). One example of this is its enhancement of PKC-dependent JNK activation (3). RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well (reviewed in 4). RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation (5).

  1. Ron, D. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 839-843.
  2. Lopez-Bergami , P. et al. (2005) Mol. Cell 19, 309-320.
  3. McCahill, A. et al. (2002) Mol. Pharmacol. 62, 1261-1273.
  4. Nilsson, J. et al. (2004) EMBO Rep. 5, 1137-1141.
  5. Chang, B. Y. et al. (2002) Oncogene 21, 7619-7629.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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