Product Pathways - Cell Cycle / Checkpoint
Aurora A/AIK (1G4) Rabbit mAb #4718
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IP IF-IC | H Mk | Endogenous | 48 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Aurora A/AIK (1G4) Rabbit mAb detects endogenous levels of total Aurora A/AIK protein.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the amino terminus of human Aurora A/AIK.
Western Blotting
Western blot analysis of extracts from HeLa, 293 and COS cells, using Aurora A/AIK (1G4) Rabbit mAb.
IF-IC
Confocal immunofluorescent images of mitotic COS cells during metaphase (left) or anaphase (right), labeled with Aurora A/AIK (1G4) Rabbit mAb (red) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Aurora A (AIK) is a cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5) and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation and altered survivin localization (6).
- Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
- Zhou, H. et al. (1998) Nat Genet 20, 189-93.
- Sen, S. et al. (1997) Oncogene 14, 2195-200.
- Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
- Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
- Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3092 Aurora A/AIK Antibody
- 3094 Aurora B/AIM1 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
