Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Snail (SN9H2) Rat mAb #4719

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 29 Rat IgG2a kappa

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Snail (SN9H2) Rat mAb detects endogenous levels of total Snail.

Source / Purification

Monoclonal antibody is produced by immunizing rats with a recombinant human Snail protein.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from HCT116, 293 cells, and 293 cells transiently transfected with a SNAIL expressing construct, using Snail (SN 9H2) Rat mAb.

Background

Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (1-3). Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines (4,5). In addition, Snail blocks the cell cycle and confers resistance to cell death (6). Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function (7-10).

  1. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-3161.
  2. Cano, A. et al. (2000) Nat. Cell Biol. 2, 76-83.
  3. Batlle, E. et al. (2000) Nat. Cell Biol. 2, 84-89.
  4. Yokoyama, K. et al. (2003) Int. J. Oncol. 22, 891-898.
  5. Yokoyama, K. et al. (2001) Oral Oncol. 37, 65-71.
  6. Vega, S. et al. (2004) Genes Dev. 18, 1131-1143.
  7. Zhou, B.P. et al. (2004) Nat. Cell Biol. 6, 931-940.
  8. Bachelder, R.E. et al. (2005) J. Cell Biol. 168, 29-33.
  9. Yook, J.I. et al. (2005) J. Biol. Chem. 280, 11740-11748.
  10. Yang, Z. et al. (2005) Cancer Res. 65, 3179-3184.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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