Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Pim-2 Antibody #4723

Applications Reactivity MW (kDa) Source
W H (Mk) 40, 38, 34 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Pim-2 Antibody detects endogenous levels of total Pim-2 protein. The antibody does not cross-react with other Pim family members.

Source / Purification

Polyclonal antibodies were prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Gln287 of human Pim-2. Antibodies were purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of recombinant Pim-1, Pim-2 and Pim-3 kinases using Pim-2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562, MOLT4 and OVCAR8 cell lines using Pim-2 Antibody.

Background

Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and is correlated with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12).

Pim-2 is highly homologous to Pim-1 with similar oncogenic functions (13,14). Three isoforms of Pim-2 can be generated from alternative start sites which run at 34, 38, and 40 kDa (13). Pim-2 leads to resistance to a variety of apoptotic stimuli and its expression is negatively regulated by growth factor withdrawal (15,16). Increased levels of Pim-2 has also been observed in certain cancers (17,18).

  1. Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15.
  2. Selten, G. et al. (1986) Cell 46, 603-11.
  3. Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12.
  4. Dautry, F. et al. (1988) J Biol Chem 263, 17615-20.
  5. Moroy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8.
  6. Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55.
  7. Leverson, J.D. et al. (1998) Mol Cell 2, 417-25.
  8. Winn, L.M. et al. (2003) Cell Cycle 2, 258-62.
  9. Pasqualucci, L. et al. (2001) Nature 412, 341-6.
  10. Kim, O. et al. (2004) Oncogene 23, 1838-44.
  11. Aho, T.L. et al. (2004) FEBS Lett 571, 43-9.
  12. Yan, B. et al. (2003) J Biol Chem 278, 45358-67.
  13. van der Lugt, N.M. et al. (1995) EMBO J. 14, 2536-2544.
  14. Breuer, M.L. et al. (1989) EMBO J. 8, 743-748.
  15. Fox, C.J. et al. (2003) Genes Dev. 17, 1841-1854.
  16. White, E. (2003) Genes Dev. 17, 1813-1816.
  17. Cohen, A.M. et al. (2004) Leuk. Lymphoma 45, 951-955.
  18. Dai, H. et al. (2005) Prostate 65, 276-286.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes