Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

TRAF2 Antibody (Human Specific) #4724

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H Mk Endogenous 53 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

TRAF2 Antibody detects endogenous levels of total human TRAF2 protein. No cross-reactivity was detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are prepared by immunizing rabbits with a synthetic peptide (KLH -coupled) corresponding to residues surrounding cysteine 192 of human TRAF2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, 293 and Jurkat cells, using TRAF2 (Human Specific) Antibody.

IF-IC

IF-IC

Immunofluorescent staining of HeLa cells showing cytoplasmic staining, using TRAF2 (Human Specific) Antibody.

Background

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation and stress responses.

While TRAF2 was originally described through its interaction with TNFRII, it has since been shown to interact with other surface receptors including CD27, CD30, CD40, 4-1BB, Ox40, HVEM/ATAR and LMP-1 (1-3). TRAF2 also associates with a large number of intracellular proteins, including TRADD, FADD, I-TRAF/TANK, TRIP, A20, c-IAP1 and 2, Casper, RIP and NIK, which help to regulate cell survival. Dominant negative and knockout studies have shown that TRAF2 plays an important role in TNF-mediated activation of NF-kB and the MAPK/JNK kinase pathway (5-7).

  1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
  2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
  3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
  4. Rothe, M. et al. (1994) Cell 78, 681-692.
  5. Yeh, W. C. et al. (1997) Immunity 7, 715-725.
  6. Reinhard, C. et al. (1997) EMBO J. 16, 1080-1092.
  7. Rothe, M. et al. (1995) Science 269, 1424-1427.

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