Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

TRAF3 Antibody #4729

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 55 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

TRAF3 Antibody detects endogenous levels of total TRAF3 protein. Cross reactivity was not detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a central region within TRAF3. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji (human), PC12 (rat), and C2C12 (mouse) cell lines, using TRAF3 Antibody.

Background

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation and stress responses.

  1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
  2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
  3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
  4. Rothe, M. et al. (1994) Cell 78, 681-692.

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