Cell Signaling Technology

Product Pathways - Apoptosis

Max Antibody #4732

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 21 to 22 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Max Antibody detects endogenous levels of total Max protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Tyr115 of human Max. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat (human), C6 (rat), and Raw (mouse) cell lines, using Max Antibody.

IP

IP

Immunoprecipitation of Jurkat cell lysates with Max Antibody or control rabbit IgG followed by Western blot analysis using Max Antibody.

Background

Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).

  1. Baudino, T.A. and Cleveland, J.L. (2001) Mol. Cell. Biol. 21, 691-702.
  2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-1217.
  3. Henriksson, M. and Lüscher, B. (1996) Adv. Cancer Res. 68, 109-182.
  4. Grandori, C. et al. (2000) Annu. Rev. Cell Dev. Biol. 16, 653-699.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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