Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

ORC2 (3G6) Rat mAb #4736

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H Mk Endogenous 78 Rat IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ORC2 (3G6) Rat mAb recognizes endogenous levels of total ORC2 protein. The antibody does not cross-react with other ORC subunits.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length recombinant human ORC2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and HUVEC cells, using ORC2 (3G6) Rat mAb.

IP

IP

Immunoprecipitation of ORC2 from Jurkat cell lysates, using ORC2 (3G6) Rat mAb followed by western blot using the same antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using ORC2 (3G6) Rat mAb (blue) and COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with DY-554 phalloidin (red).


Background

The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7).

  1. Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624-27630.
  2. Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428-12435.
  3. Gibson, D.G. et al. (2006) Genes Cells 11, 557-573.
  4. Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763-776.
  5. Rowles, A. and Blow, J.J. (1997) Curr. Opin. Genet. Dev. 7, 152-157.
  6. DePamphilis, M.L. (2003) Gene 310, 1-15.
  7. McNairn, A.J. et al. (2005) Exp. Cell. Res. 308, 345-356.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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