Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

ORC6 (3A4) Rat mAb #4737

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Hm Mk Endogenous 28 Rat IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ORC6 (3A4) Rat mab recognizes endogenous levels ot total ORC6 protein. The antibody does not cross-react with other ORC subunits.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full length recombinant human ORC6.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using ORC6 (3A4) Rat mAb.

IP

IP

Immunoprecipitation of ORC6 from Jurkat cell lysates followed by Western blot, using ORC6 (3A4) Rat mAb.

Background

The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7).

  1. Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624-27630.
  2. Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428-12435.
  3. Gibson, D.G. et al. (2006) Genes Cells 11, 557-573.
  4. Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763-776.
  5. Rowles, A. and Blow, J.J. (1997) Curr. Opin. Genet. Dev. 7, 152-157.
  6. DePamphilis, M.L. (2003) Gene 310, 1-15.
  7. McNairn, A.J. et al. (2005) Exp. Cell. Res. 308, 345-356.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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