Product Pathways - Apoptosis / Autophagy
Max (S20) Antibody #4739
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R (Mk) (B) | Endogenous | 19-21 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Max (S20) Antibody detects endogenous levels of total Max protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to residues near the amino terminus of Max. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with human Max contruct, using Max (S20) Antibody.
Western Blotting
Western blot analysis of extracts from various cell lines using Max (S20) Antibody.
IF-IC
Confocal immunofluorescent analysis of ACHN cells using Max (S20) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).
- Baudino, T.A. and Cleveland, J.L. (2001) Mol. Cell. Biol. 21, 691-702.
- Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-1217.
- Henriksson, M. and Luscher, B. (1996) Adv. Cancer Res. 68, 109-182.
- Grandori, C. et al. (2000) Annu. Rev. Cell Dev. Biol. 16, 653-699.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
