Cell Signaling Technology

Product Pathways - MAPK Signaling

RKIP Antibody #4742

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk (B) Endogenous 21 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RKIP Antibody detects endogenous levels of total RKIP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to human and mouse RKIP. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS, C6, NIH/3T3, HeLa and A431 cells, using RKIP Antibody.

Background

Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-κB pathway, including NF-κB-inducing kinase (NIK), TAK1, IKKα and IKKβ (5). These interactions antagonize cytokine-induced activation of the NF-κB pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6).

  1. Yeung, K. et al. (1999) Nature 401, 173-7.
  2. Yeung, K. et al. (2000) Mol Cell Biol 20, 3079-85.
  3. Corbit, K.C. et al. (2003) J Biol Chem 278, 13061-8.
  4. Lorenz, K. et al. (2003) Nature 426, 574-9.
  5. Yeung, K.C. et al. (2001) Mol Cell Biol 21, 7207-17.
  6. Fu, Z. et al. (2003) J Natl Cancer Inst 95, 878-89.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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