Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

TRAF6 Antibody #4743

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 60 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

TRAF6 Antibody detects endogenous levels of total TRAF6 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a sythetic peptide (KLH-coupled) corresponding to resides near the amino terminus of human TRAF6. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock-transfected or transfected with human TRAF6, using TRAF6 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 and Jurkat cell lines using TRAF6 Antibody.

Background

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation and stress responses.

TRAF6 plays a critical role in innate and adaptive immunity, bone metabolism, and development of certain tissues including the nervous system (5).TRAF6 deficiency results in osteopetrosis and defective IL-1, CD40, and LPS signaling (6) as well as defects in neuronal development (7). Unlike other TRAF family members that mediate signaling through TNF, TRAF6 has unique binding activities (8) that results in signaling responses from the interleukin-1 receptor (IL-1R) (9), toll-like receptor (10,11). CD40 (12), RANK (13,14), and p75 neurtrophin receptor (15). TRAF6 associates directly with CD40 and RANK, and indirectly with IL-1R/TLR through IRAK (10). It leads to activation of NF-κB and MAP kinase signaling pathways though downstream association with the TAB/TAK-1 complex (16). TRAF6 also activates Src family nonreceptor tyrosine kinases leading to Akt activation (17).

  1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
  2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
  3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
  4. Rothe, M. et al. (1994) Cell 78, 681-692.
  5. Wu, H. and Arron, J.R. (2003) Bioessays 25, 1096-1105.
  6. Lomaga, M.A. et al. (1999) Genes Dev. 13, 1015-1024.
  7. Lomaga, M.A. et al. (2000) J. Neurosci. 20, 7384-7393.
  8. Ye, H. et al. (2002) Nature 418, 443-447.
  9. Cao, Z. et al. (1996) Nature 383, 443-446.
  10. Muzio, M. et al. (1997) Science 278, 1612-1615.
  11. Medzhitov, R. et al. (1998) Mol .Cell 2, 253-258.
  12. Ishida, T. et al. (1996) J. Biol. Chem. 271, 28745-28748.
  13. Darnay, B.G. et al. (1998) J. Biol. Chem. 273, 20551-20555.
  14. Wong, B.R. et al. (1998) J. Biol. Chem. 273, 28355-28359.
  15. Khursigara, G. et al. (1999) J. Biol. Chem. 274, 2597-2600.
  16. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
  17. Wong, B.R. et al. (1999) Mol. Cell 4, 1041-1049.

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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