Cell Signaling Technology

Product Pathways - Development

TRA-1-60(S) (TRA-1-60(S)) Mouse mAb #4746

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC F H Endogenous 200-400 Mouse IgM

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen.

Source / Purification

Monoclonal antibody is produced by immunizing animals with human embryonic carcinoma 2102Ep cl.2A6 cells.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TRA-1-60 (TRA-1-60) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA2 cells (green) using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of nTERA2 cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6).

  1. Andrews, P.W. et al. (1987) Int J Androl 10, 95-104.
  2. Andrews, P.W. et al. (1991) Recent Results Cancer Res 123, 63-83.
  3. Badcock, G. et al. (1999) Cancer Res 59, 4715-9.
  4. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30.
  5. Thomson, J.A. et al. (1998) Science 282, 1145-7.
  6. Marrink, J. et al. (1991) Int J Cancer 49, 368-72.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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