Cell Signaling Technology

Product Pathways - Development

TRA-2-54 (2J) Mouse mAb #4747

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 80 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TRA-2-54 (2J) Mouse mAb recognizes endogenous levels of total TRA-2-54 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with 2102Ep human embryonal carcinoma cells.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TRA-2-54 (2J) Mouse mAb.

Background

The family of alkaline phosphatases in humans is comprised of four members: intestinal, placental, placental-like (germ cell type), and tissue nonspecific. The tissue nonspecific isozyme is also known as the liver/bone/kidney alkaline phosphatase due to its expression in these tissues. It is also highly expressed in embryonic stem (ES) and embryonic carcinoma (EC) cells and is lost as these cells undergo differentiation (1,2). The TRA-2-54 (2J) Mouse mAb specifically detects this isozyme and does not detect the other family members, making it a useful tool for tracking the pluripotency status of ES and EC cells in culture.

  1. Andrews, P.W. et al. (1996) Int J Cancer 66, 806-16.
  2. Draper, J.S. et al. (2002) J Anat 200, 249-58.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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