Product Pathways - Adhesion
Integrin Antibody Sampler Kit #4749
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Integrin α4 Antibody #4600||40 µl||W IP||H||70, 140, 150, (180)||Rabbit|
|Integrin α5 Antibody #4705||40 µl||W||H M Mk||150||Rabbit|
|Integrin αV Antibody #4711||40 µl||W IP||H R||135, 140||Rabbit|
|Integrin β1 Antibody #4706||40 µl||W||H M R Mk||115, 135||Rabbit|
|Integrin β3 Antibody #4702||40 µl||W||H M R Mk B||97, 110, 130||Rabbit|
|Integrin β4 Antibody #4707||40 µl||W||H||210||Rabbit|
|Integrin β5 Antibody #4708||40 µl||W IP IF-IC||H||90||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
All antibodies in the Integrin Antibody Sampler Kit detect endogenous levels of their target α and β integrin subunits of the integrin dimer. None of the antibodies provided detect intact dimers or their activational state. Integrin α4 Antibody detects endogenous levels of integrin α4 mature protein (150kDa), α4 precursor protein (140kDa), as well as the 70 kDa cleaved C-terminal α4 fragment.
Western blot analysis of extracts from Jurkat and MOLT-4 cells using Integrin α4 Antibody #4600.
Western blot analysis of extracts from various cell lines using Integrin β3 Antibody #4702.
Western blot analysis of extracts from A549, NIH/3T3 and COS cell lines using Integrin α5 Antibody #4705.
Western blot analysis of extracts from various cell lines using Integrin β1 Antibody #4706.
Western blot analysis of extracts from various cell lines using Integrin β4 Antibody #4707.
Western blot analysis of extracts from HT29 and A172 cells using Integrin β5 Antibody #4708.
The Integrin Antibody Sampler Kit provides an economical means to screen samples for α and β subunits of integrin molecules. The kit includes enough primary and secondary antibody to perform four Western blot experiments with each antibody.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1027 of human integrin α4, Ser458 of human integrin α5 protein, Arg108 of human integrin αV, Pro680 of human integrin β1 protein, human and mouse integrin β3, Ser1812 of human integrin β4 protein and Lys789 of human integrin β5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).
- Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
- Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
- van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 9999 Nonfat Dry Milk
- 9998 BSA
For Research Use Only. Not For Use In Diagnostic Procedures.