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Integrin Antibody Sampler Kit #4749

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
Integrin α4 Antibody #4600	40 µl	W IP	H	70, 140, 150, (180)	Rabbit
Integrin α5 Antibody #4705	40 µl	W	H M Mk	150	Rabbit
Integrin αV Antibody #4711	40 µl	W IP	H R	135, 140	Rabbit
Integrin β1 Antibody #4706	40 µl	W	H M R Mk	115, 135	Rabbit
Integrin β3 Antibody #4702	40 µl	W	H M R Mk B	97, 110, 130	Rabbit
Integrin β4 Antibody #4707	40 µl	W	H	210	Rabbit
Integrin β5 Antibody #4708	40 µl	W IP IF-IC	H	90	Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

All antibodies in the Integrin Antibody Sampler Kit detect endogenous levels of their target α and β integrin subunits of the integrin dimer. None of the antibodies provided detect intact dimers or their activational state. Integrin α4 Antibody detects endogenous levels of integrin α4 mature protein (150kDa), α4 precursor protein (140kDa), as well as the 70 kDa cleaved C-terminal α4 fragment.

Description

The Integrin Antibody Sampler Kit provides an economical means to screen samples for α and β subunits of integrin molecules. The kit includes enough primary and secondary antibody to perform four Western blot experiments with each antibody.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1027 of human integrin α4, Ser458 of human integrin α5 protein, Arg108 of human integrin αV, Pro680 of human integrin β1 protein, human and mouse integrin β3, Ser1812 of human integrin β4 protein and Lys789 of human integrin β5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).

1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

4600:

Immunoprecipitation, Western Blotting

4702:

Western Blotting

4705:

Western Blotting

4706:

Western Blotting

4707:

Western Blotting

4708:

Immunofluorescence, Immunoprecipitation, Western Blotting

4711:

Immunoprecipitation, Western Blotting

Companion Products

• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7075 Anti-biotin, HRP-linked Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 9999 Nonfat Dry Milk
• 9998 BSA

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For Research Use Only. Not For Use In Diagnostic Procedures.