Cell Signaling Technology

Product Pathways - Phosphatases

DUSP3/VHR Antibody #4752

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 20 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DUSP3/VHR Antibody detects endogenous levels of total DUSP3 protein. This antibody does not cross-react with other dual-specific phosphatases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human DUSP3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using DUSP3/VHR Antibody.

Background

DUSP3, also known as VHR (VH1 related) is a small dual-specific phosphatase with specificity for MAP kinase ERK1/2 and JNK, but not for p38 MAPK (1,2). Unlike most of the dual-specific phosphatases, which have inducible expression patterns, DUSP3 is constitutively expressed (2). In antigen stimulated T cells, DUSP3 is phosphorylated by ZAP-70 at Tyr138 (3). Tyr138 phosphorylation is required for DUSP3 to down-regulate the ERK and JNK pathways (3).

  1. Todd, J. L. et al. (1999) J. Biol. Chem. 274, 13271-13280.
  2. Alonso, A. et al. (2001) J. Biol. Chem. 276, 4766-4771.
  3. Alonso, A. et al. (2002) Nat. Immunol. 4, 44-48.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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