Cell Signaling Technology

Product Pathways - Antibody Sampler Kits

Cellular Localization IF Antibody Sampler Kit #4753

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
β-Tubulin (9F3) Rabbit mAb #2128 40 µl W IHC-P IF-IC F H M R Mk Z B (C) 55 Rabbit IgG
COX IV (3E11) Rabbit mAb #4850 40 µl W IP IHC-P IHC-F IF-IC F H R Mk Z B Pg 17 Rabbit IgG
NUP98 (C39A3) Rabbit mAb #2598 40 µl W IP IF-IC H M R Mk 98 Rabbit IgG
CENP-A Antibody #2186 40 µl W IF-IC H 17 Rabbit
Fibrillarin (C13C3) Rabbit mAb #2639 40 µl W IF-IC H M R Mk 37 Rabbit IgG
LC3B (D11) XP® Rabbit mAb #3868 40 µl W IP IHC-P IF-IC F H M R (Mk) (B) (Pg) 14, 16 Rabbit IgG
Rab5 (C8B1) Rabbit mAb #3547 40 µl W IF-IC H M R Mk 25 Rabbit IgG
Calnexin (C5C9) Rabbit mAb #2679 40 µl W IHC-P IF-IC H Mk 90 Rabbit
Histone H3 (D1H2) XP® Rabbit mAb #4499 40 µl W IHC-P IF-IC H M R Mk (Hm) (C) (Dm) (X) (Z) (B) 17 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Dm=D. melanogaster  X=Xenopus  Z=Zebrafish  B=Bovine  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the Cellular Localization IF Antibody Sampler Kit recognizes only it specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using β-Tubulin (9F3) Rabbit mAb #2128 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using CENP-A Antibody #2186 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of Caki-1 cells using NUP98 (C39A3) Rabbit mAb #2598 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Fibrillarin (C13C3) Rabbit mAb #2639 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Calnexin (C5C9) Rabbit mAb #2679 (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Rab5 (C8B1) Rabbit mAb #3547 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or chloroquine-treated (right), using LC3B (D11) XP® Rabbit mAb #3868 (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Histone H3 (D1H2) XP® Rabbit mAb #4499 (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ™ (fluorescent DNA dye).


Description

The Cellular Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immnuocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four Western blots with each antibody.

Source / Purification

Rabbit monoclonal and polyclonal antibodies are prepared by immunizing animals with a synthetic peptide corresponding to: the amino terminus of human β-tubulin, the sequence of human GAPDH, the sequence of human calnexin, residues surrounding Lys29 of human COX IV, residues surrounding Gly190 of human Rab5 protein, the carboxy-terminal sequence of human histone H3, residues surrounding Pro671 of human NUP98, residues surrounding Thr298 of human fibrillarin, residues near the amino terminus of LC3B, and human CENP-A protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

* Product-specific protocol.

Companion Products

Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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