Product Pathways - Antibody Sampler Kits
Cellular Localization IF Antibody Sampler Kit #4753
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| β-Tubulin (9F3) Rabbit mAb # 2128 | 40 microliters | W IHC-P IF-IC F | H M R Mk B Z (C) | 55 | Rabbit IgG |
| Calnexin Antibody # 2433 | 40 microliters | W IHC-P IF-IC | H | 90 | Rabbit |
| COX IV (3E11) Rabbit mAb # 4850 | 40 microliters | W IP IHC-P IHC-F IF-IC F | H R Mk Pg B Z | 17 | Rabbit IgG |
| Rab5 Antibody # 2143 | 40 microliters | W IF-IC | H M R Mk Hm | 25 | Rabbit |
| Histone H3 Antibody # 9715 | 40 microliters | W IP IHC-P IF-IC | H M R Mk Pg B Z (Dm) | 17 | Rabbit |
| NUP98 (C39A3) Rabbit mAb # 2598 | 40 microliters | W IP IF-IC | H M R Mk | 98 | Rabbit IgG |
| Atg12 Antibody (Human Specific) # 2010 | 40 microliters | W IP IF-IC | H | 16, 53 | Rabbit |
| CENP-A Antibody # 2186 | 40 microliters | W IF-IC | H | 17 | Rabbit |
| Fibrillarin (C13C3) Rabbit mAb # 2639 | 40 microliters | W IF-IC | H M R Mk | 37 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Pg=Pig
C=Chicken
Hm=Hamster
B=Bovine
Dm=D. melanogaster
Z=Zebrafish
Specificity / Sensitivity
Each antibody in the Cellular Localization IF Antibody Sampler Kit recognizes only it specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.
IF-IC
Confocal immunofluorescent analysis of chloroquine-treated HCT-116 cells using Atg12 Antibody (Human Specific) (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using β-Tubulin (9F3) Rabbit mAb #2128 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells using Rab5 Antibody #2143 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using CENP-A Antibody #2186 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Calnexin Antibody #2433 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorecent DNA dye).
IF-IC
Confocal immunofluorescent analysis of Caki-1 cells using NUP98 (C39A3) Rabbit mAb #2598 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Fibrillarin (C13C3) Rabbit mAb #2639 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-IC
Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ™ (fluorescent DNA dye).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Histone H3 Antibody #9715 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Description
The Cellular Localization IF Antibody Sampler Kit provides an economical means for identification cellular organelles by fluorescence immnuocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four Western blots with each antibody.
Source / Purification
Rabbit monoclonal and polyclonal antibodies are prepared by immunizing animals with a synthetic peptide corresponding to: the amino terminus of human β-tubulin, the sequence of human GAPDH, the sequence surrounding Ala51 of human calnexin, residues surrounding Lys29 of human COX IV, residues surrounding Gly190 of human Rab5 protein, the carboxy-terminal sequence of human histone H3, residues surrounding Pro671 of human NUP98, residues surrounding Thr298 of human fibrillarin, residues near the amino terminus of human Atg12, and human CENP-A protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Background
Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 2010:
- Immunofluorescence, Immunoprecipitation, Western Blotting
- 2128:
- Flow, IHC / Paraffin, Immunofluorescence, Western Blotting
- 2143:
- Immunofluorescence, Western Blotting
- 2186:
- Immunofluorescence, Western Blotting
- 2433:
- IHC / Paraffin, Immunofluorescence, Western Blotting
- 2598:
- Immunofluorescence, Immunoprecipitation, Western Blotting
- 2639:
- Immunofluorescence, Western Blotting
- 4850:
- Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
- 9715:
- IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
Companion Products
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 9998 BSA
- 9999 Nonfat Dry Milk
- 4084 DRAQ5®
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
