Product Pathways - Development
SSEA4 (MC813) Mouse mAb #4755
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IF-IC F | H | Endogenous | N/A | Mouse IgG3 |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4755:
- Flow*, Immunofluorescence*
* Product-specific protocol.
Specificity / Sensitivity
SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen.
Source / Purification
Monoclonal antibody is produced by injecting animals with human embryonal carcinoma 2102Ep cl.2A6 cells.
Flow Cytometry
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA cells (green) using SSEA4 (MC813) Mouse mAb.
IF-IC
Confocal immunofluorescent analysis of NTERA2 cells using SSEA4 (MC813) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
SSEA4 (stage-specific embryonic antigen 4) is a glycolipid carbohydrate epitope expressed on the surface of human teratocarcinoma stem cells, human embryonic germ cells, and human embryonic stem cells (1). Expression of human SSEA4 decreases following differentiation of human embryonal carcinoma cells. Expression of the SSEA4 antigen is absent in murine pluripotent cells, but increases following differentiation (1,2).
Application References
- Krupnick, J.G. et al. (1994) Int J Cancer 59, 692-8. Applications: Fluorescent Detection IC-IF
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Companion Products
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For Research Use Only. Not For Use In Diagnostic Procedures.
