Product Pathways - Apoptosis / Autophagy
DcR1 Antibody #4756
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 28 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
DcR1 detects endogenous levels of total DcR1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy terminus of human DcR1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat, Raji and A20 cell lines, using DcR1 Antibody.
Background
The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5 and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC) resulting in activation of caspases.
Death receptor signaling is also controlled by a family of decoy receptors (DcR1, DcR2 and DcR3) which lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand (3-5). Expression of decoy receptors provide a mechanism for certain types of cancer to regulate apoptosis and can contribute to chemosensitivity (6-8).
- Nagata, S. (1997) Cell 88, 355-365.
- Thorburn, A. (2004) Cell. Signal. 16, 139-144.
- Sheridan, J.P. et al. (1997) Science 277, 818-821.
- Marsters, S.A. et al. (1997) Curr. Biol. 7, 1003-1006.
- Pitti, R.M. et al. (1998) Nature 396, 699-703.
- Liu, X. et al. (2005) Cancer Res. 65, 9169-9175.
- Spalding, A.C. et al. (2002) Oncogene 21, 260-271.
- Bernard, D. et al. (2001) J. Biol. Chem. 276, 27322-27328.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
