Product Pathways - Tyrosine Kinase / Adaptors
Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb #4757
PhosphoSitePlus® protein, site, and accession data: HER4
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H (M) (R) | Endogenous | 180 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4757:
- Western Blotting
Specificity / Sensitivity
Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr1284. This antibody does not cross-react with other ErbB family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1284 of human HER4/ErbB4.
Western Blotting
Western blot analysis of extracts from various cell lines expressing different activated protein tyrosine kinases, using Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower).
Background
The HER/ErbB receptor tyrosine kinase family has been implicated in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).
- Holbro, T. and Hynes, N.E. (2004) Annu Rev Pharmacol Toxicol 44, 195-217.
- Roskoski, R. (2004) Biochem Biophys Res Commun 319, 1-11.
- Carpenter, G. (2003) Exp Cell Res 284, 66-77.
Application References
- Weigelt, B. et al. (2010) Breast Cancer Res Treat 122, 35-43. Applications: Western Blotting
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Companion Products
- 4795 HER4/ErbB4 (111B2) Rabbit mAb
- 3790 Phospho-HER4/ErbB4 (Tyr984) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
