Cell Signaling Technology

Product Pathways - Apoptosis

DcR3 Antibody #4758

Applications Reactivity Sensitivity MW (kDa) Source
W H R Endogenous 32 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DcR3 Antibody detects endogenous levels of total DcR3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DcR3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of lysates from LN18, HeLa and HCT116 cell lines, using DcR3 Antibody.

Background

The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases.

Death receptor signaling is also controlled by a family of decoy receptors (DcR1, DcR2 and DcR3) which lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand (3-5). Expression of decoy receptors provide a mechanism for certain types of cancer to regulate apoptosis and can contribute to chemosensitivity (6-8).

  1. Nagata, S. (1997) Cell 88, 355-365.
  2. Thorburn, A. (2004) Cell. Signal. 16, 139-144.
  3. Sheridan, J.P. et al. (1997) Science 277, 818-821.
  4. Marsters, S.A. et al. (1997) Curr. Biol. 7, 1003-1006.
  5. Pitti, R.M. et al. (1998) Nature 396, 699-703.
  6. Liu, X. et al. (2005) Cancer Res. 65, 9169-9175.
  7. Spalding, A.C. et al. (2002) Oncogene 21, 260-271.
  8. Bernard, D. et al. (2001) J. Biol. Chem. 276, 27322-27328.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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