Cell Signaling Technology

Product Pathways - Translational Control

ER Protein Folding Antibody Sampler Kit #4759

Kit Includes Quantity Applications Reactivity MW (kDa) Source
BiP (C50B12) Rabbit mAb # 3177 40 microliters W IHC-P IHC-F H M 78 Rabbit
Ero1-Lα Antibody # 3264 40 microliters W H 60 Rabbit
ERp44 Antibody # 2886 40 microliters W H M R Mk 44 Rabbit
ERp57 (G117) Antibody # 2881 40 microliters W H M R 57 Rabbit
ERp72 Antibody # 2798 40 microliters W H M R Mk 72 Rabbit
Grp94 Antibody # 2104 40 microliters W H M R Mk 100 Rabbit
PDI Antibody # 2446 40 microliters W IHC-P IF-IC H M R Mk 57 Rabbit
Anti-rabbit IgG, HRP-linked Antibody # 7074 100 microliters Goat

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey

Specificity / Sensitivity

Each antibody in the ER Protein Folding Antibody Sampler Kit detects endogenous levels of its target protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Grp94 Antibody #2104.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using PDI Antibody #2446.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ERp72 Antibody #2798.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ERp57 (G117) Antibody #2881.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ERp44 Antibody #2886.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using BiP (C50B12) Rabbit mAb #3177.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Ero1-Lα Antibody #3264.

Source / Purification

Rabbit monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Gly584 of human BiP. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide (KLH-coupled) derived from the sequence of human ERp44, the sequences around Gly117 of human ERp57, Pro329 of human PDI, Met622 of human Grp94, Leu218 of human Ero1-Lα, and the sequence of human ERp72. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

After their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins (3). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress proteins 72, 57 and 44 (ERp72, ERp57 and ERp44), constitute the PDI family (4,5,6). The ER also contains a pool of molecular chaperones, including Grp94, to help proteins fold properly. Grp94 is a glucose-regulated protein (7) with sequence homology to Hsp90 (8). BiP is another chaperone whose synthesis is increased when protein folding is disturbed. BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (9).

  1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
  2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
  3. Tu, B.P. and Weissman, J.S. (2004) J Cell Biol 164, 341-6.
  4. Mazzarella, R.A. et al. (1990) J Biol Chem 265, 1094-101.
  5. Satoh, M. et al. (2005) Cell Stress Chaperones 10, 278-84.
  6. Jessop, C.E. et al. (2007) EMBO J 26, 28-40.
  7. Lee, A.S. et al. (1981) Proc Natl Acad Sci U S A 78, 4922-5.
  8. Sorger, P.K. and Pelham, H.R. (1987) J Mol Biol 194, 341-4.
  9. Kohno, K. et al. (1993) Mol Cell Biol 13, 877-90.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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