Product Pathways - Translational Control
ER Protein Folding Antibody Sampler Kit #4759
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|BiP (C50B12) Rabbit mAb #3177||40 µl||W IHC-P IHC-F||H M||78||Rabbit IgG|
|Ero1-Lα Antibody #3264||40 µl||W||H||60||Rabbit|
|ERp44 (D17A6) XP® Rabbit mAb #3798||40 µl||W IHC-P||H M R Mk||44||Rabbit IgG|
|ERp57 (G117) Antibody #2881||40 µl||W||H M R||57||Rabbit|
|ERp72 (D70D12) XP® Rabbit mAb #5033||40 µl||W IF-IC F||H M R Mk||72||Rabbit IgG|
|Grp94 Antibody #2104||40 µl||W||H M R Mk||100||Rabbit|
|PDI (C81H6) Rabbit mAb #3501||40 µl||W IHC-P IF-IC||H M R Mk||57||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the ER Protein Folding Antibody Sampler Kit detects endogenous levels of its target protein.
Western blot analysis of extracts from various cell types using Grp94 Antibody #2104.
Western blot analysis of extracts from various cell types using ERp57 (G117) Antibody #2881.
Western blot analysis of extracts from various cell types using BiP (C50B12) Rabbit mAb #3177.
Western blot analysis of extracts from various cell types using Ero1-Lα Antibody #3264.
Western blot analysis of extracts from various cell lines using PDI (C81H6) Rabbit mAb #3501.
Western blot analysis of extracts from various cell lines using ERp44 (D17A6) XP® Rabbit mAb #3798.
The ER Protein Folding Antibody Sampler Kit contains reagents to investigate the initiation of translation within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Gly584 of human BiP, the sequence of human ERp44, the residues surrounding Met279 of human ERp72 protein and the sequence of human PD1. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequences around Gly117 of human ERp57, Met622 of human Grp94 and Leu218 of human Ero1-Lα. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
After their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins (3). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress proteins 72, 57 and 44 (ERp72, ERp57 and ERp44), constitute the PDI family (4,5,6). The ER also contains a pool of molecular chaperones, including Grp94, to help proteins fold properly. Grp94 is a glucose-regulated protein (7) with sequence homology to Hsp90 (8). BiP is another chaperone whose synthesis is increased when protein folding is disturbed. BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (9).
- Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
- Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
- Tu, B.P. and Weissman, J.S. (2004) J Cell Biol 164, 341-6.
- Mazzarella, R.A. et al. (1990) J Biol Chem 265, 1094-101.
- Satoh, M. et al. (2005) Cell Stress Chaperones 10, 278-84.
- Jessop, C.E. et al. (2007) EMBO J 26, 28-40.
- Lee, A.S. et al. (1981) Proc Natl Acad Sci U S A 78, 4922-5.
- Sorger, P.K. and Pelham, H.R. (1987) J Mol Biol 194, 341-4.
- Kohno, K. et al. (1993) Mol Cell Biol 13, 877-90.
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* Product-specific protocol.
- 3177 BiP (C50B12) Rabbit mAb
- 3183 BiP Antibody
- 3264 Ero1-Lα Antibody
- 2886 ERp44 Antibody
- 2881 ERp57 (G117) Antibody
- 2887 ERp57 (A484) Antibody
- 2798 ERp72 Antibody
- 2104 Grp94 Antibody
- 2446 PDI Antibody
- 9956 ER Stress Antibody Sampler Kit
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.