Product Pathways - Neuroscience
Nestin (Rat-401) Mouse mAb #4760
PhosphoSitePlus® protein, site, and accession data: nestin
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IHC-P IF-F | R | Endogenous | 207 | Mouse IgG1 |
Applications Key:
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4760:
- IHC / Paraffin, Immunofluorescence
Specificity / Sensitivity
Nestin (Rat-401) Mouse mAb detects endogenous levels of nestin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.
Background
Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and is generally accepted as a marker for neural cancer stem cells (3). Nestin expression has alos been observed in astrocytes, retina, cardiac muscle, pancreas and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.
- Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
- Giliarov, A.V. (2007) Morfologiia 131, 85-90.
- Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.
Application References
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Companion Products
- 8112 SignalStain® Antibody Diluent
- 2837 Neurofilament-L (C28E10) Rabbit mAb
- 2835 Neurofilament-L (DA2) Mouse mAb
- 2836 Neurofilament-H (RMdO 20) Mouse mAb
- 2838 Neurofilament-M (RMO 14.9) Mouse mAb
- 4084 DRAQ5®
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.