Product Pathways - Neuroscience
Nestin (Rat-401) Mouse mAb #4760
PhosphoSitePlus® protein, site, and accession data: nestin
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IHC-P IF-F | R | Endogenous | 207 | Mouse IgG1 |
Applications Key:
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4760:
- IHC / Paraffin, Immunofluorescence
Specificity / Sensitivity
Nestin (Rat-401) Mouse mAb detects endogenous levels of nestin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat brain, P1 (left) or P14 (right), using Nestin (Rat-401) Mouse mAb (top) or Neurofilament-L (C28E10) Rabbit mAb #2837 (lower). Note decreased Nestin staining as NFL staining increases in the developing rat brain.
IF-F
Confocal immunofluorescent analysis of P1 rat brain (left) and adult rat brain (right) using Nestin (Rat-401) Mouse mAb (green) and Neurofilament-L (C28E10) Rabbit mAb #2837 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.
- Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
- Giliarov, A.V. (2007) Morfologiia 131, 85-90.
- Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 8112 SignalStain® Antibody Diluent
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
- 2837 Neurofilament-L (C28E10) Rabbit mAb
- 2835 Neurofilament-L (DA2) Mouse mAb
- 2836 Neurofilament-H (RMdO 20) Mouse mAb
- 2838 Neurofilament-M (RMO 14.9) Mouse mAb
- 4084 DRAQ5®
For Research Use Only. Not For Use In Diagnostic Procedures.
