Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Filamin A (Ser2152) Antibody #4761

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 280 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Phospho-Filamin A (Ser2152) Antibody detects endogenous levels of filamin A only when phosphorylated at serine 2152. This antibody also reacts with filamin C when it is phosphorylated at serine 2146.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser2152 of human filamin A and Ser2146 of human filamin C. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 and HeLa cells, untreated or lamda phosphatase-treated, using Phospho-Filamin A (Ser2152) Antibody (upper) and Filamin A Antibody #4762 (lower).

Background

Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (alpha-actinin domain) is located at the very amino-terminus, followed by as many as 24 tandem repeats of about 96 residues, and the dimerization domain is located at the carboxy-terminal end of th protein. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta-integrins, Rho/Rac/Cdc42, PKC, and insulin receptor, primarily through their carboxy-terminal half (1-3). Filamin A, the most abundant, and filamin B, less abundant, are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Serine 2152 of filamin A is phosphorylated by PAK1, and this phosphorylation is required for PAK1-mediated actin cytoskeleton reorganization (4).

  1. Stossel, T. P. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 138-145.
  2. Tigges, U. et al. (2003) J. Biol. Chem. 278, 23561-23569.
  3. He, H. et al. (2003) J. Biol. Chem. 278, 27096-27104.
  4. Vadlamudi, R. K. et al. (2002) Nat. Cell Biol. 4, 681-690.

Application References

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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