Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Filamin A (Ser2152) Antibody #4761

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 280 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Filamin A (Ser2152) Antibody detects endogenous levels of filamin A only when phosphorylated at serine 2152. This antibody also reacts with filamin C when it is phosphorylated at serine 2146.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser2152 of human filamin A and Ser2146 of human filamin C. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 and HeLa cells, untreated or lamda phosphatase-treated, using Phospho-Filamin A (Ser2152) Antibody (upper) and Filamin A Antibody #4762 (lower).

Background

Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (alpha-actinin domain) located at the amino terminus is followed by as many as 24 tandem repeats of about 96 residues and the dimerization domain is located at the carboxy terminus. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta integrins, Rho/Rac/Cdc42, PKC, and the insulin receptor, primarily through the carboxy-terminal dimerization domain (1-3). Filamin A, the most abundant, and filamin B are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Filamin A is phosphorylated by PAK1 at Ser2152, which is required for PAK1-mediated actin cytoskeleton reorganization (4).

  1. Stossel, T. P. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 138-145.
  2. Tigges, U. et al. (2003) J. Biol. Chem. 278, 23561-23569.
  3. He, H. et al. (2003) J. Biol. Chem. 278, 27096-27104.
  4. Vadlamudi, R. K. et al. (2002) Nat. Cell Biol. 4, 681-690.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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